Controlled Light Exposure Microscopy (CLEM):
نویسنده
چکیده
Introduction • Methods
منابع مشابه
Controlled Light Exposure Microscopy (CLEM): Photobleaching and Phototoxicity Reduced
Controlled Light Exposure Microscopy (CLEM): Photobleaching and Phototoxicity Reduced Photobleaching and phototoxicity are often the Achilles‘ heels of fluorescence live-cell imaging. Controlled Light Exposure Microscopy (CLEM) is a novel, simple imaging approach that reduces photobleaching and phototoxicity 2 to 10-fold without compromising image quality. The basic concept of CLEM is that ligh...
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Correlative light/electron microscopy (CLEM) combines the power of light microscopy to search large areas for fluorescently-tagged live cells/regions of interest and transmission electron microscopy (TEM) to image the same specimen at high magnification for ultrastructural characterization. CLEM methods are essential to observe dynamic processes such as organelle biogenesis and intracellular tr...
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Correlative light and electron microscopy (CLEM) is an effective technique used to study biological samples. Signal-specific indicators observable under light microscopy (LM) allow scientists to locate areas of interest for high resolution ultra-structure observation under the electron microscope (EM). Recent method developments provided breakthroughs creating an effective, direct, and accurate...
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Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasib...
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Fluorescence Microscopy (FM) localization of GFP derivatives provides information – sometimes dynamic about the distribution of proteins in cells and tissues while electron microscopy provides information about the ultrastructural components of cells. Correlative light and electron microscopy (CLEM) integrates these two complementary imaging techniques generating a more complete view of the mic...
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تاریخ انتشار 2007